| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.030323 |
| Chromosome: | chromosome 12 |
| Location: | 9752728 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551977 | PHC74 | Putative pherophorin-chlamydomonas homolog; (1 of 1) 2.7.11.1//3.6.5.5 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dynamin GTPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAAACAGCGCATAGGTGCAGTCGGCGCCCGAGCCATAGCACAAGCGC |
| Internal bar code: | GGTCCCAATCTAGAACAGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2003 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTCTCAACTTCCTGCTG |
| Suggested primer 2: | ATCAACTAGCCCACCACTGC |