Insertion junction: CLIP2.030328_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.030328
Chromosome:chromosome 2
Location:4612258
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g107350 DHC4 (1 of 3) IPR003593//IPR004273//IPR011704//IPR013602//IPR024317//IPR024743//IPR026983//IPR027417 - AAA+ ATPase domain // Dynein heavy chain domain // ATPase, dynein-related, AAA domain // Dynein heavy chain, domain-2 // Dynein heavy chain, P-loop containing D4 domain // Dynein heavy chain, coiled coil stalk // Dynein heavy chain // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGATGGCGTCCACCGGGCCGTCCAGCACCAGCCACTTGCGGTCGGGCG
Internal bar code:GATCACGCTTGACTCCTTGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1920
LEAP-Seq percent confirming:43.1818
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGTGTCATGACTTGGGA
Suggested primer 2:TGTGACTACGTGACGGGTTG