Insertion junction: CLIP2.030353_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030353
Chromosome:chromosome 7
Location:2608043
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g330150 (1 of 7) IPR011335 - Restriction endonuclease type II-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTTTCCGCTCGCCGCGTCCCCGGACGCCATCATCGCTCACCACCTGC
Internal bar code:GATCTGCTTATGTCCGGGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5506
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTCCTCCTCCTCCTCC
Suggested primer 2:CCAGGAAGGTTAGTGCACGT