| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.030358 |
| Chromosome: | chromosome 1 |
| Location: | 842413 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004700 | (1 of 7) PF03188 - Eukaryotic cytochrome b561 (Cytochrom_B561) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGCTTCTCTGGGTGAGAGCGGGCACAGACTCGGCATGTAGGCTACCT |
| Internal bar code: | ACGGGGTGGTGTAGGACTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 86.5385 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGAGGTACTTGGCCTGGC |
| Suggested primer 2: | AATGTGCGCTGATGTGGGTA |