Insertion junction: CLIP2.030371_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.030371
Chromosome:chromosome 17
Location:264425
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697650 (1 of 1) K01354 - oligopeptidase B (ptrB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATCTTCTCTGAGCGGCTCTTGCCCACGCCCACGTAGAAGGCCTCGTC
Internal bar code:ACTAGGCGTGGACGACCGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:71
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACAACGCCACCCTGTTCTA
Suggested primer 2:CCACACTGTACTCCGTGTCC