Insertion junction: CLIP2.030371_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.030371
Chromosome:chromosome 17
Location:264430
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697650 (1 of 1) K01354 - oligopeptidase B (ptrB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGCAGCGGTGAGGCGGGGGGAGGGGAGGCGAGGGGGAAGGGGGTTTGC
Internal bar code:ACTAGGCGTGGACGACCGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:96
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACTGTACTCCGTGTCC
Suggested primer 2:GACAACGCCACCCTGTTCTA