| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.030401 |
| Chromosome: | chromosome 8 |
| Location: | 4392264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385050 | MAPK2 | Mitogen-Activated Protein Kinase Homolog 2; (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGGGAAGGGGCCGTGGGAGGGCTACGCGAGCTCACACGCAGGGCCT |
| Internal bar code: | TTACGGTTAGGTTCCACGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACGACGACCTGACTCCG |
| Suggested primer 2: | GACATGCCAAAACACCCCAC |