Insertion junction: CLIP2.030416_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.030416
Chromosome:chromosome 6
Location:7901829
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g303950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACCTGATGCACCTGGGCGCCTTCCGCCCCTTCGAGTGCCTGCCGCGC
Internal bar code:CGGAGGGTTGTAGAACAGCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:86
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AACGGAGAGGTCTAGCGAGT
Suggested primer 2:GCTGGTCTAGGCTAGCTTCG