| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.030441 |
| Chromosome: | chromosome 8 |
| Location: | 589230 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359900 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCGTGCAGTTTGGCGGCGCGCCCGCCATGCCCAGCCCGTTGGGGC |
| Internal bar code: | AATGGCGCTTTATACAATGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2706 |
| LEAP-Seq percent confirming: | 72.4138 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACAGTCCCGTGTTGCTG |
| Suggested primer 2: | CGGAGGCAGTGGTATGCTAC |