Insertion junction: CLIP2.030446_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.030446
Chromosome:chromosome 9
Location:1498854
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399601 (1 of 15) IPR029057 - Phosphoribosyltransferase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTTCGCCACACGGGGCCGAGTTTGGGGTTCGCGGCAACGACATATGTC
Internal bar code:ACAGTAGTTGGTTGGTCCCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:4188
LEAP-Seq percent confirming:31.8182
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCTTTTTGTCACCACAG
Suggested primer 2:CCATGACGCGATGAAAGCTG