| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.030447 |
| Chromosome: | chromosome 3 |
| Location: | 5336391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183000 | intron | ||
| lncRNA_TCONS_00234526 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAGGGTTACCGTGGGTCCCAGGACACGGCCTCACCCGGTGCGTGTGC |
| Internal bar code: | TTTCAGCAGTAAGATACATGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4063 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGAGCACACGCCGTATG |
| Suggested primer 2: | CATAACCTGCGCATTGGAGC |