| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030455 |
| Chromosome: | chromosome 14 |
| Location: | 1199324 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615650 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGCAAGCCTACTAGAGCTAGAGTAGGCCGACGGCGTTGCAGGCGGC |
| Internal bar code: | GGGTCGCAGAGGAGACGCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1319 |
| LEAP-Seq percent confirming: | 67.7419 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACAATGCCCTGACGGT |
| Suggested primer 2: | TGTATGAACACGGCGGGAAA |