Insertion junction: CLIP2.030458_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.030458
Chromosome:chromosome 1
Location:2307666
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g012900 (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCATCCGCTGCCGCGACCGGCAGAAGCTGCTGTTCGACACGGTGTGCA
Internal bar code:CAGAAAAACAATGGGCAAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:882
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATACAATCATTTGCGCCCGC
Suggested primer 2:AGCCTCTGAATCGGAGTCCT