| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.030459 |
| Chromosome: | chromosome 10 |
| Location: | 4241766 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449650 | (1 of 4) IPR001810//IPR020683 - F-box domain // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGCATGACTGTCTGGAGCAGCTGTGCCGCTGGGGACACCTGCGGGT |
| Internal bar code: | GTTTTGGACCGGAAGACAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1215 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTGAATGCCACCGAACA |
| Suggested primer 2: | CGTGAGGTTATGTGGGCCTT |