| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030476 |
| Chromosome: | chromosome 12 |
| Location: | 4627994 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522050 | (1 of 1) PTHR11851//PTHR11851:SF140 - METALLOPROTEASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTAATTAAGGGGGGACATTCATGATTAGAAGGCGTAGCCAGCAACAGG |
| Internal bar code: | TTGCACTCAGGAAGGCGTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGACCGTTGCTGTTGTTG |
| Suggested primer 2: | CCACAGTGATGGAGGACACC |