| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030500 |
| Chromosome: | chromosome 17 |
| Location: | 4337762 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730300 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | 5'UTR | |
| Cre17.g802084 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGCAGCTGGCTGTGAGTGGTCCGCGGCGCCGCTGGAGGCGGCCATGC |
| Internal bar code: | GGGGCATGTAGATTGAGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3066 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCAAGCAGGTAGGCCAG |
| Suggested primer 2: | CATGTGCGTATGGACTTGCG |