| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.030504 |
| Chromosome: | chromosome 8 |
| Location: | 551642 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359600 | CSE22 | Predicted protein of CSE family | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCTAACCATCAAACCACATCATGTGCGGTCTTGATTGATTGATTTGC |
| Internal bar code: | TGGTAGCGGGGAGTGTATCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1427 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGGTTGAGTCTGTTACG |
| Suggested primer 2: | GCGTTGTTGATGCTGGTGTT |