| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.030550 |
| Chromosome: | chromosome 10 |
| Location: | 1904763 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431450 | NAT24 | N-acetyltransferase; (1 of 1) 2.3.1.1//2.3.1.88 - Amino-acid N-acetyltransferase / N-acetylglutamate synthase // Peptide alpha-N-acetyltransferase / Protein N-terminal acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAACCGAGAAGCGCTCTGCAAGCAGGCACCAGGGGCTCTCCGTAAAC |
| Internal bar code: | TCCTTCAAGATTAGTATTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3085 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAATGGTGTCTGGACGTG |
| Suggested primer 2: | CATCAAGCCGGTAACCCAGT |