| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.030560 |
| Chromosome: | chromosome 3 |
| Location: | 1750105 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCATGCACTTGTGTGAGGGGACAAGTGCGGGACAGGAACAAACATACA |
| Internal bar code: | TGAAAGTTCTATGAAATGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 37.2549 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCTTGATGCATGCCAC |
| Suggested primer 2: | TACGCATGACACGTGTGACA |