| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.030562 |
| Chromosome: | chromosome 5 |
| Location: | 500556 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244200 | TMA1,COTH2 | spore coat protein H; (1 of 3) PF08757 - CotH protein (CotH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATGCTCCCGTACCTTGACCGCTAATGTTCGTTGACTGCAGGGGCGG |
| Internal bar code: | ATAAGTCGTCAGTCATTCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATGTGTCATCGCTGAG |
| Suggested primer 2: | TTATGTGGGAGCTTGTGCGT |