Insertion junction: CLIP2.030590_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.030590
Chromosome:chromosome 14
Location:819579
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g612850 (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 CDS
Cre14.g612950 CYG17 (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACGTCACCGTACCCAATATCTGCGCCCGCTCTGCCACTCGGTCAGCGG
Internal bar code:GCGGGATGCTCAGTTTTTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:876
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CATCGGCGTGATCAGCAATG
Suggested primer 2:ACCAGGAAGAAAGAGGCGTG