Insertion junction: CLIP2.030624_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.030624
Chromosome:chromosome 15
Location:5645237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGTCCATCGTAGGCACACATACGTGCGGGGGTGGCGCCCGACCTTAG
Internal bar code:TTTTTTTGGAGAATTATGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1745
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGCTTCCTGCTCCCTC
Suggested primer 2:GCGTTTATGGTGTGAGGGGA