Insertion junction: CLIP2.030634_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.030634
Chromosome:chromosome 10
Location:2564717
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g436500 XPP SurE-like phosphatase/nucleotidase; (1 of 1) PTHR30457:SF0 - 5'/3'-NUCLEOTIDASE SURE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTAACGCCGCCAACCAGCAATGGATTATGTTTGCTGCGCTGATCCATG
Internal bar code:TTGACAGGGGTTGATGATTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2037
LEAP-Seq percent confirming:96.7742
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGGAAACTGCACACTGC
Suggested primer 2:GCCGCTAATCATCAGGTCGA