| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.030653 |
| Chromosome: | chromosome 2 |
| Location: | 3279436 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097400 | EIF5A | (1 of 1) K03263 - translation initiation factor 5A (EIF5A); Eukaryotic initiation factor, eIF-5A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGAAGGCCAGGCCAGGGCGGAAACAGACACAGGGAGCGTAGGGCCC |
| Internal bar code: | GTTTACAAATGACATACGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2822 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGCATCAGGAGCAAGG |
| Suggested primer 2: | TTGGCCACCTCACACTGTTT |