| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030659 |
| Chromosome: | chromosome 4 |
| Location: | 1154251 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215750 | (1 of 1) K02180 - cell cycle arrest protein BUB3 (BUB3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCAACCCTTGCCCCTCTTGCCCCCCGCTGCACTGCACAGGGTGAGCG |
| Internal bar code: | CGATTTTTACGATTCGGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCCTTTGCACGTTCATG |
| Suggested primer 2: | TCCGACTCTTTTTCCCTGCC |