Insertion junction: CLIP2.030669_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030669
Chromosome:chromosome 4
Location:2362661
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g221100 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCGGCACCAAAATGCGAGAAGACGAGAACTGAGCATGCACAGAATGC
Internal bar code:ACGGTCATACGTAACGAGGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3218
LEAP-Seq percent confirming:98.7952
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:GGAACACAAAGTCACACCGC
Suggested primer 2:TTTCACTCCGTCACTCTGCC