Insertion junction: CLIP2.030670_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030670
Chromosome:chromosome 10
Location:2628173
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g437050 ATP11 (1 of 1) K07555 - ATP synthase mitochondrial F1 complex assembly factor 1 (ATPeAF1, ATPAF1, ATP11); Assembly factor 1 for F1 component of mitochondrial ATP synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCAGCTCCAGACAGATGCACGGGGGACATAGCATGGTGAGCACATGTC
Internal bar code:GATCCCTTGGTTCAATCTCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1879
LEAP-Seq percent confirming:63.4146
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTGCCTTTGATACCGCG
Suggested primer 2:CTCAAGGCCTGTGATGAGCA