Insertion junction: CLIP2.030691_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.030691
Chromosome:chromosome 3
Location:6579044
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g194600 (1 of 2) IPR000719//IPR011009//IPR013221 - Protein kinase domain // Protein kinase-like domain // Mur ligase, central intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGATGCTTAACTCAGCCTCGCGCGGACGTTGGACGAGCGAGTCGCGCTG
Internal bar code:TGGTTTCGACGGTTATTGTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4442
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGATCCACCTCCATCTGG
Suggested primer 2:CGGCCAATTGTCCGCAATAG