Insertion junction: CLIP2.030692_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.030692
Chromosome:chromosome 10
Location:2830276
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g439000 (1 of 3) 3.6.3.47 - Fatty-acyl-CoA-transporting ATPase; Putative ABC transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGTTGACGCTCTCGGCATTTGATGTACCCTTACCTGCACACGCCCCG
Internal bar code:CGGAATGCAGGTGGGCAATAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:211
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATCACGTGCTAGAAGCG
Suggested primer 2:CTCCACCAGCACCCATACTG