| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030725 |
| Chromosome: | chromosome 10 |
| Location: | 6187791 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462816 | (1 of 2) IPR007112//IPR007117//IPR009009 - Expansin/pollen allergen, DPBB domain // Expansin, cellulose-binding-like domain // RlpA-like double-psi beta-barrel domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACGAGGATGGGAATGGGCGACGGGAGGGGCGGGAAGGGAAAGGGGG |
| Internal bar code: | TGGGTTCGCGGTGCCGGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCCCAACGAGATAGGTC |
| Suggested primer 2: | ATGAGTCCCGCGAATACGTC |