| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.030740 |
| Chromosome: | chromosome 4 |
| Location: | 2844575 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224200 | (1 of 2) PTHR18841//PTHR18841:SF0 - VITELLINE MEMBRANE OUTER LAYER PROTEIN I-RELATED // PROTEIN C08G5.3, ISOFORM A-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGTGGCCTGCTGCCAGCGGGGCGCAAACCGCGACGCGCCATAAGCA |
| Internal bar code: | CGTGTAGGGGCTATTAGGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1347 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAAAATGGGAGCGCTAAG |
| Suggested primer 2: | CAATGTGCCCCAGTACTCGT |