Insertion junction: CLIP2.030741_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.030741
Chromosome:chromosome 10
Location:4000409
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g447350 SEC23B (1 of 1) PF04810//PF04811 - Sec23/Sec24 zinc finger (zf-Sec23_Sec24) // Sec23/Sec24 trunk domain (Sec23_trunk); COP-II coat subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGTCAGGTTGGTCTGCAGGGGCCACCAAGGCGCCGCACAGGCACACCG
Internal bar code:TTTAGACGGTATATCTCCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2150
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCCGAGGGATTGGCTTG
Suggested primer 2:GGAGGGTGAGCACAAGTGAA