| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030762 |
| Chromosome: | chromosome 17 |
| Location: | 6667336 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745947 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACGGTCACAGCCGCGGCCGCGGTCTTCGTAGTGTGAGGGTGGCGGCA |
| Internal bar code: | CATCAAGCTGCGTAAAGACAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4155 |
| LEAP-Seq percent confirming: | 10.4167 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGCCTCAGTCAAGGAT |
| Suggested primer 2: | ATGGGTGTCAGGGGGTCATA |