| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.030763 |
| Chromosome: | chromosome 12 |
| Location: | 5579144 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531000 | AMT1H,AMT8 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGAGCGCACCAGCCTGGTGGCCTACCCCGTGTTCACGGTGCTCATG |
| Internal bar code: | ATATTGCGTGTATACTGACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCACAGGAAGCTGCAAGT |
| Suggested primer 2: | ATACGGCCGCTCTAAGAACG |