Insertion junction: CLIP2.030776_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.030776
Chromosome:chromosome 12
Location:6663773
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCGAGGTTTTACCAATCAGCCAACCCATACCAATCCTGACCAATGCT
Internal bar code:GATATTGTTCGCGAACACTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3333
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CATGACTAAATTGCCCGCGG
Suggested primer 2:CTTTGGCACACCTTGACACG