Insertion junction: CLIP2.030777_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.030777
Chromosome:chromosome 6
Location:3735809
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278162 PHC3 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACAGGGCGTACTGCACGGAGGTCGGCGACATGAACAGCTCGCTGATC
Internal bar code:ACAGTAATCACCCCAGGGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1871
LEAP-Seq percent confirming:95.3488
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTCCTCTTCCCTCTTAC
Suggested primer 2:GGGGTCAACGTACAGCTTCA