| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030779 |
| Chromosome: | chromosome 16 |
| Location: | 7008205 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673393 | (1 of 2) PTHR13710//PTHR13710:SF81 - DNA HELICASE RECQ FAMILY MEMBER // ATP-DEPENDENT DNA HELICASE Q-LIKE 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTTTGCTGTGGATGAGGCGCACACGGTAAGCTGCCATTCTACCGTAC |
| Internal bar code: | GACACGCCCCAGTCAAACTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2838 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTGAAGGAGCCCTTGAT |
| Suggested primer 2: | GCTTGGGGTGGGGTAAGTAC |