| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030788 |
| Chromosome: | chromosome 14 |
| Location: | 216808 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609030 | (1 of 3) 3.5.5.1 - Nitrilase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTATCGCGGGTAAGTATGACTACCTGGGTGACGACAACCACGCGACGC |
| Internal bar code: | CCCTCCATCTATCAATTATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAATGCACGGGACTTG |
| Suggested primer 2: | TCCGTCTCACGTTCTTTGGG |