Insertion junction: CLIP2.030792_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030792
Chromosome:chromosome 13
Location:3850344
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCACTGCCTGCCCTCCACACACAGGTTCCGCGCCGTGTTTGGAGACCG
Internal bar code:GCCAGTCTAAACAAACTTGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:866
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAGGACGTAGGTCACCT
Suggested primer 2:CTACATGCAAACGAGCGCTC