Insertion junction: CLIP2.030805_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030805
Chromosome:chromosome 2
Location:2986778
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095400 BRIX1 Ribosome biogenesis protein; (1 of 1) K14820 - ribosome biogenesis protein BRX1 (BRX1, BRIX1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAGGATGGTCCCGGGGGATGTCTGCTGGCCCGACGAAACCCTGCTCCC
Internal bar code:GTCTGTGTTAGTTCGCGCATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1526
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCACGCTCTGAGGAAACA
Suggested primer 2:ACACCGCGTGTTGACAGTAT