| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.030812 |
| Chromosome: | chromosome 14 |
| Location: | 1152507 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615100 | MSR1,MSRB1B | (1 of 3) K07305 - peptide-methionine (R)-S-oxide reductase (msrB); Methionine sulphoxide reductase 1B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTTACAAGTCGTGACGCGCTTTTAAGTTGTGACGCGTTGAACCAGCG |
| Internal bar code: | TTCCTCGTAATGTCGAGAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 56.25 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTCGGAGCAGTCAACAA |
| Suggested primer 2: | GATCGCATCGGTGTCTGGAT |