Insertion junction: CLIP2.030812_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.030812
Chromosome:chromosome 14
Location:1152517
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g615100 MSR1,MSRB1B (1 of 3) K07305 - peptide-methionine (R)-S-oxide reductase (msrB); Methionine sulphoxide reductase 1B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTAAGCTAATATAGACGAATCCGGAGAGCGCAGCCTTCCGCGCAGGGA
Internal bar code:TTCCTCGTAATGTCGAGAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2094
LEAP-Seq percent confirming:98.9247
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:93

Suggested primers for confirmation by PCR

Suggested primer 1:GATCGCATCGGTGTCTGGAT
Suggested primer 2:GAGGTCGGAGCAGTCAACAA