| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.030821 |
| Chromosome: | chromosome 9 |
| Location: | 1321794 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400550 | NOA1 | Nitric oxide associated protein; (1 of 1) K13427 - nitric-oxide synthase, plant (NOA1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGAAGGACGGTGGCGTTGTCACGCCACAACAGTGCACCAGCGGACGT |
| Internal bar code: | TAATGGGGGGACAGTTTAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCCCCCAAAACCCAAA |
| Suggested primer 2: | AGGAGGAGCAGAGAGTCAGG |