Insertion junction: CLIP2.030836_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.030836
Chromosome:chromosome 12
Location:2160814
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g509500 (1 of 1) IPR000595//IPR002048//IPR011992//IPR018490//IPR029530 - Cyclic nucleotide-binding domain // EF-hand domain // EF-hand domain pair // Cyclic nucleotide-binding-like // Cell division control protein 31 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCTTCGATACTCCATCCCCACCACCTCGACCTCGCCCTGCAGCCCCA
Internal bar code:TGATTACAGGAACTCACCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:3174
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGTCGCGCTCTCTCGAA
Suggested primer 2:AGCTTATTGGAGATCCGGCG