Insertion junction: CLIP2.030849_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.030849
Chromosome:chromosome 1
Location:3886992
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025551 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCTTGGCGGCGCCCTTGCTCGCCGGCGGCGGCGCTGCCGCGGCATTG
Internal bar code:GGAATTTTATGTATTTACTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2117
LEAP-Seq percent confirming:89.4737
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGTCCTGTGTTTGCTGC
Suggested primer 2:CGTCCAGGTCGTTGAAGTCA