Insertion junction: CLIP2.030854_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.030854
Chromosome:chromosome 17
Location:1916272
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709900 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTGAAGGCCATCAAGTGAGCGCGGGGCATAGTGTAACTTGTTTGTTGT
Internal bar code:GCAAAATCATCGATTGCTTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2509
LEAP-Seq percent confirming:97.0149
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAAAGGTAGACACGCCCC
Suggested primer 2:CCAACCAGCTTCAGCACAAG