Insertion junction: CLIP2.030861_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.030861
Chromosome:chromosome 2
Location:7679993
Confidence (%):80
Locus systematic id Locus common name Defline Feature
lncRNA_TCONS_00136852

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTGTACGCACCATCAAACAAGGTTGATTGTAACAAAAGCATGCGACCC
Internal bar code:TTAAAATGAAAATGCGCGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1772
LEAP-Seq percent confirming:97.561
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGGGCTGAAACAACATTT
Suggested primer 2:GTCCAGGGGAGGCACTACTA