Insertion junction: CLIP2.030866_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030866
Chromosome:chromosome 14
Location:2683650
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g801640 (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCCAAACCTGCCATCCCACTTACCCACACAGTCCCGCAGTGTTGGCA
Internal bar code:TTCTGATCATTCAGGGTTATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2261
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCGCAGCAATTAGGTTC
Suggested primer 2:CTTGCAAGGGAAAAGTGGGC