| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.030868 |
| Chromosome: | chromosome 6 |
| Location: | 3038018 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274650 | NUOAF4 | (1 of 3) IPR008979//IPR013857 - Galactose-binding domain-like // NADH:ubiquinone oxidoreductase intermediate-associated protein 30; Complex I intermediate-associated CIA30 protein, mitochondrial | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCAACCACCGCCGCAGCCCCCTCGCCGGGCACCGCGGGGTTCATCTGG |
| Internal bar code: | AACTTGTTATCAGAGACATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1329 |
| LEAP-Seq percent confirming: | 63.0435 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGAGACTCACTCGCCGAA |
| Suggested primer 2: | TCACTCCCTTCTAGCTCCCC |