| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.030878 |
| Chromosome: | chromosome 12 |
| Location: | 1346589 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489250 | (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCGCAGCTGCTGGCGCTGCGGCGCCAGGAGGCTCTCATGCAGCTGGA |
| Internal bar code: | CATATAGGAACGTGACTTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1785 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGACACGGTACAGCCTG |
| Suggested primer 2: | TCGCCCACATGGTAATGCTT |